phluc reporter plasmids Search Results


phluc reporter plasmids  (Addgene inc)
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Addgene inc phluc reporter plasmids
FIGURE 1 | Overview of the <t>pHLuc</t> system and experimental workflow. The <t>pHLuc</t> <t>reporter</t> is a multicistronic cassette that comprises of cytoplasmic Antares, T2A-puromycin, a mutated internal ribosome entry site (IRES) v24, and a membrane-bound SEPLuc that utilizes a weaker variant of Nanoluc. Cells transfected with pHLuc have shown that the reporter is pH-responsive compared to a pH-stable control. Since the SEP signal is pH-dependent, the ratio of SEP to Antares (R580/510) is indicative of changes in extracellular pH. When cells stably expressing pHLuc were injected into mice, bioluminescence imaging also showed differences in the ratiometric readout. In vivo ratiometric images showed the capacity of pHLuc to detect extracellular pH changes. Image generated via BioRender.
Phluc Reporter Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 1 | Overview of the pHLuc system and experimental workflow. The pHLuc reporter is a multicistronic cassette that comprises of cytoplasmic Antares, T2A-puromycin, a mutated internal ribosome entry site (IRES) v24, and a membrane-bound SEPLuc that utilizes a weaker variant of Nanoluc. Cells transfected with pHLuc have shown that the reporter is pH-responsive compared to a pH-stable control. Since the SEP signal is pH-dependent, the ratio of SEP to Antares (R580/510) is indicative of changes in extracellular pH. When cells stably expressing pHLuc were injected into mice, bioluminescence imaging also showed differences in the ratiometric readout. In vivo ratiometric images showed the capacity of pHLuc to detect extracellular pH changes. Image generated via BioRender.

Journal: Frontiers in bioengineering and biotechnology

Article Title: pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis.

doi: 10.3389/fbioe.2020.00412

Figure Lengend Snippet: FIGURE 1 | Overview of the pHLuc system and experimental workflow. The pHLuc reporter is a multicistronic cassette that comprises of cytoplasmic Antares, T2A-puromycin, a mutated internal ribosome entry site (IRES) v24, and a membrane-bound SEPLuc that utilizes a weaker variant of Nanoluc. Cells transfected with pHLuc have shown that the reporter is pH-responsive compared to a pH-stable control. Since the SEP signal is pH-dependent, the ratio of SEP to Antares (R580/510) is indicative of changes in extracellular pH. When cells stably expressing pHLuc were injected into mice, bioluminescence imaging also showed differences in the ratiometric readout. In vivo ratiometric images showed the capacity of pHLuc to detect extracellular pH changes. Image generated via BioRender.

Article Snippet: The pHluc reporter plasmids described here are deposited with Addgene and are available upon request, from Addgene (Supplementary Table 1).

Techniques: Membrane, Variant Assay, Transfection, Control, Stable Transfection, Expressing, Injection, Imaging, In Vivo, Generated